Домівка

№ 766 (2013)

Шклярський В.І., Матієшин Ю.М., Баланюк Ю.В., Гудзь Б.В. Точність визначення параметрів двох динамічних мікрооб’єктів за допомогою телевізійного сканувального оптичного мікроскопа

УДК 621.397+681.723

В.І. Шклярський, Ю.М. Матієшин, Ю.В. Баланюк, Б.В. Гудзь
Національний університет “Львівська політехніка”
ТОЧНІСТЬ ВИЗНАЧЕННЯ ПАРАМЕТРІВ ДВОХ ДИНАМІЧНИХ МІКРООБ’ЄКТІВ ЗА ДОПОМОГОЮ ТЕЛЕВІЗІЙНОГО СКАНУВАЛЬНОГО ОПТИЧНОГО МІКРОСКОПА
© Шклярський В.І., Матієшин Ю.М., Баланюк Ю.В., Гудзь Б.В., 2013

V.I. Shkliarskyi, Yu.M. Matiieshyn, Yu.V. Balanyuk, B.V. Hudz
Lviv Polytechnic National University
ACCURACY OF DETERMINATION OF TWO DYNAMIC MICROOBJECTS PARAMETERS WITH TELEVISION SCANNING OPTICAL MICROSCOPE
© Shkliarskyi V.I., Matiieshyn Yu.M., Balanyuk Yu.V., Hudz B.V., 2013

At the present stage of technological development there are more problems associated with micro- and nanotechnology. Among such problems occupy an important place task analysis of dynamic processes and several dynamic microobjects (MO) parameters simultaneously in view of the microscope in different fields (medicine, microbiology, ecology, microelectronics etc.). Identification number of dynamic parameters (for example, mobility) of some living cells and their components occurs mainly by using optical microscopy methods. To determine the mobility is most often used in two ways: 1) the method of fluorescence microphotographs analysis; 2) the method of quantitative fluorescence confocal laser scanning microscopy. Using these methods were quantitatively and qualitatively describes the process of intracellular transport, particularly cytoplasm-nuclear, kinetic accumulation in the nucleus of cells of different types of proteins, localization of drugs aimed at developing ways to intracellular delivery of anticancer drugs. To solve these and many such important tasks in the fields of medicine and microbiology can be successfully used television scanning optical microscope (TSOM) based on cathode ray tubes ultrahigh resolution, as provided in this scan resolution up to 0.1 ... 0.2 micron. This article contains analysis of the capabilities of this microscope in the case of the simultaneous study of several MO, which are move randomly - with variable speed and direction of motion. The basic principles of TSOM work in determining the dynamic parameters of separate MO and averaged parameters of several MO are submitted. Track each individual MO occurs by the use of scan mode of miniraster, the center of which the current frame is formed with coordinates that correspond to coordinates of the center of the MO in the previous frame scan. Interval of definition of coordinate position of each MO in sight of TSOM corresponds to the duration of one frame scanning at a constant speed scanning. Dimensions of the scanning raster should be reduced to the values 1...10 % of the full-scale raster, while receiving miniraster. However, a scan should be used for MO of proportionate size with minimal scanning spot that move randomly at high speed. Among the MO for which the use of the scanning mode is not appropriate, you can select one or several MO simultaneously with large size (5...40 % of the full-scale raster in the plane of study). For such MO should be used only scanning with a full-scale square raster. Based on these considerations, we construct a mathematical model of the scanning process and analyze the significance of dynamic parameters which can be determined by TSOM using scan mode with a full-scale raster and tracking each MO by miniraster.
Key words: television scanning optical microscopy, parameters of microobjects group, scanning miniraster.

Розглядаються питання використання телевізійного сканувального оптичного мікроскопа для визначення різних параметрів динамічних мікрооб’єктів, які перебувають у групі, із застосуванням режимів сканування повноформатним растром та слідкування за кожним окремим мікрооб’єктом за допомогою сканувального мінірастра. Наведені принципи роботи мікроскопа, що забезпечують визначення параметрів як окремих динамічних мікрооб’єктів, так і групи загалом.
Ключові слова: телевізійна сканувальна оптична мікроскопія, параметри групи динамічних мікрооб’єктів, сканувальний мінірастр.

Кількість посилань 5

Янкевич Р.В., Мартюгов С.О., Токарчук П.А., Міськів М.В., Огородник А.В. Моделювання передавача SOQPSK-TG сигналів

УДК 621.396.96

Р.В. Янкевич, С.О. Мартюгов, П.А. Токарчук, М.В. Міськів, А.В. Огородник
Національний університет “Львівська політехніка”
МОДЕЛЮВАННЯ ПЕРЕДАВАЧА SOQPSK-TG СИГНАЛІВ
© Янкевич Р.В., Мартюгов С.О., Токарчук П.А., Міськів М.В., Огородник А.В., 2013

R.V. Yankevych, S.O. Martyugov, P.A. Tokarchuk, M.V. Miskiv, A.V. Ogorodnyk
Lviv Polytechnic National University
MODELLING OF TRANSMITTER OF SOQPSK-TG SIGNALS
© Yankevych R.V., Martyugov S.O., Tokarchuk P.A., Miskiv M.V., Ogorodnyk A.V., 2013

This paper is devoted to the practical side of the SOQPSK transmitter realization. It describes detailed model of one of possible variants of transmitter realization. The model is made in such a way that any part of it can be easily changed or any parameter can be tuned to achieve best efficiency of the system.
The biggest problem to be solved when realizing transmitter is practical realization of ternary encoder. This paper also presents our solution of this problem using elemental discrete blocks that could be easily realized in practice either using discrete elements or included as part of system realized on FPGA.
The paper itself is divided on four sections:
1. Introduction. This part contains the information about the development of telemetry systems, standard types of modulations and references links to the papers devoted to the problems of telemetry signals.
2. SOQPSK modulation and its kinds. This section contains theoretical statements and formulas of the SOQPSK type modulation. Also different parameters that define types of SOPQSK modulation (SOQPSK-A, SOQPSK-B, SOQPSK-TG) are presented.
3. Transmitter model. In this section the problem of telemetry SOQPSK-TG transmitter modeling is described. Next the step by step explanation of the model, its blocks and their operation is shown. Explanation is organized in the way how the input signal propagates from input to output. Also the problem of transmitter filter is described. Two possible solutions of this issue are shown in this section too.
4. Conclusions. Some short conclusions to this paper are given in this section.
In general we can say that the necessity of new telemetry systems creation force the search for new, more effective modulations. Created standards describe some of the signals types and their parameters. But these stands do not fully describe methods of these signals generation. Within the work on creation of detailed model of SOQPSK transmitter the scheme of binary sequences of {-1, +1} alphabet to ternary sequences of {-1, 0, +1} alphabet was created. The created model allows to investigate the influence of its separate blocks’ parameters change on the whole system, but also easily make these changes. Also, it is necessary to point out that such model detail allows to make relatively easy practical realization just by transferring it to FPGA, which in now a days have big enough library of standard blocks and systems.
Key words: Telemetry, SOQPSK signals, ternary converters.

Описано процес моделювання, а також результати моделювання передавального пристрою SOQPSK-TG сигналів. Представлено реалізацію двійково-трійкового перетворювача.
Ключові слова: телеметрія, SOQPSK сигнали, трійкові перетворювачі.

Кількість посилань 10

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